Schematic of the active zone
of
the
frog's neuromuscular
junction
inferred from models
generated by
EM3D.
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Success Stories
EM3D's scheme for the automatic alignment of tilt-images is described.
Ress, D., Harlow, M.L., Schwarz, M., Marshall, R.M., and U.J. Mc Mahan. Automatic acquisition of fiducial markers and alignment of images in tilt series for electron tomography.
J. Electron Microscopy 48: 277-287, 1999
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EM3D is first used for exposing celluar architecture at macromolecular resolution.
Harlow, M.L., Ress, D., Stoschek, A., Marshall, R.M. and McMahan, U.J. The architecture of active zone material at the frog's neuromuscular junction.
Nature 409:479-484, 2001 |
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EM3D's method for creating optimal isodensity surface models is described.
Ress, D., Harlow, M.L., Marshall, R.A., and McMahan, U.J. Optimized Method for Isodensity Surface Models Obtained with Electron Microscope Tomography Data. Engineering in Medicine and Biology Society, 2003. Proceedings of the 25th Annual International Conference of IEEE. pp. 774-777, 2003. |
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EM3D is used to examine the architecture of the postsynaptic density at brain synapses.
Petersen, J.D., Chen, X., Vinada, L., Dosemeci, A., Lisman, J.E., and Reese, T.S. Distribution of postsynaptic density (PSD)-95 and Ca2+/calmodulin-dependent protein kinase II at the PSD.
J. Neuroscience, 23: 11270-11278, 2003.
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EM3D approach to model generation is detailed.
Ress, D.B., Harlow, M.L., Marshall, R.M. and McMahan, U.J. Methods for generating high-resolution structural models from electron microscope tomography data.
Structure: 12 (10):1763-1774, 2004.
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