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Click on image for larger view.

Schematic of the active zone of the
frog's neuromuscular junction
inferred from models generated by
EM3D.


 

Success Stories


EM3D's scheme for the automatic alignment of tilt-images is described.
Ress, D., Harlow, M.L., Schwarz, M., Marshall, R.M., and U.J. Mc Mahan. Automatic acquisition of fiducial markers and alignment of images in tilt series for electron tomography.
J. Electron Microscopy 48: 277-287, 1999

 

EM3D is first used for exposing celluar architecture at macromolecular resolution.

Harlow, M.L., Ress, D., Stoschek, A., Marshall, R.M. and McMahan, U.J. The architecture of active zone material at the frog's neuromuscular junction.
Nature
409:479-484, 2001
 

EM3D's method for creating optimal isodensity surface models is described.

Ress, D., Harlow, M.L., Marshall, R.A., and McMahan, U.J. Optimized Method for Isodensity Surface Models Obtained with Electron Microscope Tomography Data. Engineering in Medicine and Biology Society, 2003. Proceedings of the 25th Annual International Conference of IEEE. pp. 774-777, 2003.
 


EM3D is used to examine the architecture of the postsynaptic density at brain synapses.

Petersen, J.D., Chen, X., Vinada, L., Dosemeci, A., Lisman, J.E., and Reese, T.S. Distribution of postsynaptic density (PSD)-95 and Ca2+/calmodulin-dependent protein kinase II at the PSD.
J. Neuroscience, 23: 11270-11278, 2003.

 


EM3D approach to model generation is detailed.

Ress, D.B., Harlow, M.L., Marshall, R.M. and McMahan, U.J. Methods for generating high-resolution structural models from electron microscope tomography data.
Structure: 12 (10):1763-1774, 2004.

 

 

 


Last modified: 5/23/07

   


This Human Brain Project/Neuroinformatics research is funded by the
National Institute of Mental Health